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- How do I interpret the red and green images?
The data for one gene corresponds to one row, and each experiment is represented by a column. The ratio of induction/repression is such that the magnitude is indicated by the intensity of the colors displayed. If the color is black then the ratio of control to experimental cDNA is equal to 1, while the brightest colors (red and green) represent a ratio of 8 to 1 or higher (ie, ratios greater than 8 are displayed as the brightest color). In all cases red indicates an increase in mRNA abundance while green indicates a decrease in abundance in the experimental sample with respect to the control. Gray areas (when visible) indicate absent data, or data of low quality.
- How is the similarity between two genes determined?
The similarity of two genes in expression space is determined by
calculating a distance measure across all experimental values. For
this we are using the Pearson correlation coefficient.
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